You only need the PCI Express 3.0 connection, the mainboard generation does not matter.

i have used the wifi card with a b450 asus board. everything works io. sometimes i suddenly lost wifi, but after i disabled hibernation for the card in the device manager it always works.

I bought the card to use it for hosted network. I have an ASUSTek Maximus VIII GENE mainboard. Did not work. That was the answer from the Gigabyte helpdesk: Only a 300 series motherboard supports the AX200. The Z170 chipset cannot support the AX200.