Yes, this SSD fits the ASUS PRIME B350M-A motherboard. The motherboard has an M.2 socket (type 2280, M Key) with PCIe 3.0 x4 support, which accepts NVMe SSDs like this one, although the full PCIe 4.0 speed is limited to PCIe 3.0. Note that the M.2 socket shares bandwidth with SATA6G_5/6.