Yes, you can transport 2 Trek Rail electric mountain bikes with this carrier, provided that the weight of each bike does not exceed 30 kg and the total load does not exceed 60 kg. This rack is designed to accommodate heavy electric bikes, with a maximum capacity of 60 kg for two bikes and a maximum weight per bike of 30 kg. It is also suitable for frames with dimensions between 22 and 80 mm and wheels up to 29 inches, which corresponds to the characteristics of Trek Rail.